| dc.description.abstract | The variation in three NADP-linked dehydrogenase enzymes; glucose-6-phosphate dehydro- genase, 6-phosphogluconate dehydrogenase and shikimate dehydrogenase as well as alpha and beta esterases was determined in 24 cultivars of Camellia sinensis and 2 other species of Camellia; C. japonica and C. irrawadiensis, using specific activity staining. The isozyme pro- files partitioned the cultivars according to their phylogenetic origins; (China, Assam, Cambodia and Japan). At all the loci studied, tea cultivars from China expressed the highest number of alleles followed by the Assam/Cambodia cultivars while the Japanese cultivars expressed the least. Camellia irrawadiensis and C. japonica showed unique isozyme profiles. The F1 progeny from an interspecific cross between C. sinensis and C. japonica displayed the normal Mendel- ian allelic segregation, while progeny from a C. sinensis and C. irrawadiensis cross displayed ‘distorted’ segregation for some alleles. Analysis of the catechin expression patterns using HPLC, also showed that Chinese teas expressed the highest number of prominent catechins while Japanese tea expressed the least. These results show that the catechin biosynthetic path- way is most diverse in China and least in Japan tea. Since the quality and pharmacological importance of tea is mainly derived from catechins and catechin precursors like the aromatic amino acids, these results have important implications in breeding strategies especially in con- nection with tea germplasm enrichment and quality. 2003 Elsevier Science Ltd. All rights reserved. | en_US |
