Superficial fungal infections are common among school-going children due to their involvement in practices that promote the spread at school or home. However, practices, knowledge and attitude of these children on prevention and control of fungal infection are poorly understood. This study assessed the practices, knowledge and attitude on the prevention and control of fungal infections among 163 children aged 5-12 years in western Kenya. A cross-sectional study design and systematic sampling method were adopted. Information on knowledge, attitude and practices on fungal prevention and control was collected using a structured questionnaire. Results showed that over 50% of the pupils had adequate knowledge on prevention and control of superficial fungal infections. On attitude, 58.9%of the children believed that those with fungal infection should not be allowed to play with others or go to school. Furthermore, 70.6% and 54.6% of the pupils agreed that sharing of toys and hairbrushes respectively leads in promoting the infections. These findings show that pupils demonstrate a good knowledge of superficial fungal with marked limited infection and control measures. Therefore, there is a need for stakeholders in health and education sectors to develop a policy framework involving learners in prevention and control of fungal infections.