Gender is used globally as a tool of planning, segregation and sometimes discrimination. Education is an enabler of universal socialization which can also be used to facilitate or prevent certain positions in life. In contemporary times development policies echo inclusivity and universality but culture continues to interface the extent to which this is attained. Objectives were; to determine the influence of education on female leadership in universities; to establish the influence of culture on female leadership in universities and to establish the influence of individual gender and leadership in universities in Kenya. Feminist and radical theories which are relevant to the study were used. The study aimed to shed light to the university management in the issue of gender balance in the employment section. This also was to boost the constitution two third gender rule implementation. The target population was 300 out of which 169 was sampled. The respondents were selected using stratified random sampling methods for both male and female senior staff. Data was collected using questionnaires and interviews. The data was analysed with the aid of SPSS software and presented in form tables. The findings from the study revealed that women are indeed under-represented in the leadership of universities in Kenya influenced by education, culture, and gender. As found in the research both public and private universities showed an aggregate mean of 3.55, SD 0.46 and 3.56 SD 0.47 in education. From the result most comments were; women lack professional confidence, low self-esteem and low education hence cannot make good leaders. Culture believes impacted more in the female leadership in the public and private universities with an aggregate mean of 3.75, SD 0.43. This was termed that women are home makers and not to be in leadership. The relationship between gender and leadership was positive and significant as indicated by r and p values (r=0.314; p :S 0.05). The recommendations made were: There should be full implementation of policies that have been put in place to empower women in all sectors of development, particularly the provisions in the constitution that prohibit appointments of more than two-thirds from either gender. Both private and public universities should strive to achieve gender equality in all their undertakings bearing in mind the legal provisions on gender issues, Transparency and meritocracy should be the only criteria used when making appointments and promotions in private and public sectors.