A culture dependant biodiversity study of extreme haloalkaliphilic bacteria from Magadi soda Lake Kenya

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dc.contributor.author Nyakeri, Evans M.
dc.contributor.author Ogola, Henry J.
dc.contributor.author Boga, Hamadi
dc.date.accessioned 2016-11-16T05:38:53Z
dc.date.available 2016-11-16T05:38:53Z
dc.date.issued 2015-06-24
dc.identifier.uri http://www.jooust. ac.ke
dc.description 1st JOOUST Scientific Conference en_US
dc.description.abstract The present study that aimed at studying the microbial ecology of Lake Magadi, isolated 37 isolates from Lake Magadi using mineral and carbon rich media types. They were characterized bycultural, biochemical and molecular approaches and screened for the ability to produce useful biotechnological enzymes and biomolecules. Screening was done by plating on basal media supplemented with the respective substrate. 34 of the isolates were Gram positive, one was Gram negative and two were Gram variable. Most of the isolates grew well at pH ranging from 6.0 – 11.0, (optimum 9.0-10.0), temperature range of 20-45oC (optimum of 30-35oC) and salinity range of 5- 30 %, optimum growth was noted at 10-15% salinity, showing that they are true haloalkalophiles. 15 isolates produced various extracellular enzymes such as amylases, lipases and proteases. Partial sequence blast analysis showed that the bacteria belonged to genera Bacillus, Clostridium and Halomonas, with relative abundances of 54%, 38% and 8% respectively. The similarity values of some of the isolates to their closest neighbours (78% 80%, 84%, 85%, 88% and 94-97%) show that they may represent new species or novel genera respectively within the lake ecosystem. The study results show that Lake Magadi harbours ecologically important micro-organisms which also have the potential for application in the biotechnology industry. en_US
dc.description.sponsorship JOOUST en_US
dc.language.iso en en_US
dc.publisher JOOUST en_US
dc.subject Haloalkalophiles en_US
dc.subject Lake Magadi en_US
dc.subject hypersaline en_US
dc.subject biotechnology en_US
dc.subject Bacillus en_US
dc.subject Clostridium en_US
dc.subject Halomonas, en_US
dc.title A culture dependant biodiversity study of extreme haloalkaliphilic bacteria from Magadi soda Lake Kenya en_US
dc.type Other en_US

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