Characterisation of the vaginal microflora of human immunodeficiency virus (HIV) positive and negative women in a sub-urban population of Kenya

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dc.contributor.author Kiama, Teresa N.
dc.contributor.author Verhelst, Rita
dc.contributor.author Mbugua, Paul M.
dc.contributor.author Vaneechoutte, Mario
dc.contributor.author Verstraelen, Hans
dc.contributor.author Estambale, Benson B.
dc.contributor.author Temmerman, Marleen
dc.date.accessioned 2018-11-09T07:40:43Z
dc.date.available 2018-11-09T07:40:43Z
dc.date.issued 2013-08-26
dc.identifier.issn 1684-5315 ©2014
dc.identifier.uri http://www.academicjournals.org/AJB
dc.identifier.uri http://ir.jooust.ac.ke:8080/xmlui/handle/123456789/2567
dc.description.abstract Lactobacilli predominate normal vaginal microflora and are important in maintenance of vaginal health. The current study set out to identify and compare culture isolates of vaginal microflora of human immunodeficiency virus (HIV) positive (HIV+ ) and HIV negative (HIV- ) women at different phases during menstrual cycle from a sub-urban population of Kenya. Seventy four (74) women, 41 HIV+ and 33 HIV- , followed up two consecutive menstrual cycles, had high vaginal swabs taken to prepare Gram stains for six visits and anaerobic cultures for four. All 751 isolates identified by t-DNA polymerase chain reaction (PCR) belong to 51 species. Species cultured more frequently in HIV+ participants were: Lactobacillus jensenii (p=0.01), Lactobacillus iners (p=0.02), Gardnerella vaginalis (p=0.01) and Peptoniphilus lacrimalis (p=0.01). Species cultured more frequently in HIV- women were Dialister micraerophilus (p=0.02) and Streptococcus agalactiae (p=0.04). Lactobacillus predominating both groups were Lactobacilli crispatus, L. jensenii, L. iners and Lactobacilli vaginalis. Bacterial vaginosis (BV) was equally high in HIV+ and HIV- women. Lactobacillus and BV-associated species were cultured more frequently in HIV+ women. Minor species differences were found. Predominant Lactobacillus in culture were L. crispatus, L. iners, L. jensenii and L. vaginalis. These women had lower concentrations of lactobacilli in vaginal microflora than observed in previous studies of Caucasian women. en_US
dc.language.iso en en_US
dc.publisher African Journal of Biotechnology en_US
dc.subject Vaginal microflora en_US
dc.subject human immunodeficiency virus (HIV) en_US
dc.subject menstrual cycle en_US
dc.subject t-DNA polymerase chain reaction (PCR) en_US
dc.subject culture en_US
dc.subject bacterial vaginosis en_US
dc.title Characterisation of the vaginal microflora of human immunodeficiency virus (HIV) positive and negative women in a sub-urban population of Kenya en_US
dc.title.alternative Characterisation of the vaginal microflora of human immunodeficiency virus (HIV) positive and negative women in a sub-urban population of Kenya en_US
dc.type Article en_US


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