The purpose of this study was to analyse the effects of implementation of prudential guidelines on profitability of Commercial Banks in Kenya, considering the sustainability of banks in Kenya, controlling for external factors, is paramount to the sustainability of banks in Kenya. The study found that risk management guidelines very highly affects the profitability of commercial, corporate governance guidelines very highly affects the profitability of commercial banks in Kenya, consumer protection guidelines highly affects the profitability of commercial banks in Kenya, and loan loss prudential guidelines very highly affects the profitability of commercial banks in Kenya. The study concludes that commercial banks in Kenya adopt prudential guidelines; risk management guidelines, corporate governance guidelines, loan loss prudential guidelines, and consumer protection guidelines to ensure considerable profitability of commercial banks in Kenya. The study recommends that the commercial banks in Kenya should; ensure strict adherence to the principles of good lending management (strict application of the prudential guidelines), ensure that the prudential guidelines and supervision are effectively followed to minimise excessive risk taking by banks, ensure presence of efficient supervision and regulation , consumers protection guidelines should ensure prompt supervision and control of loan accounts, should be used for professional valuation of properties being offered as collaterals and perfecting their legal mortgages through the loan loss prudential guidelines.